Table 3 siRNAs designed in the present study for the “N” gene and various parameters
siRNA | Conserved region ID | Target sequence (21 + 2 nt) | siRNA sequence (antisense/guide) 21 nt | Sense/passenger (19 nt) | SMEpred (efficacy) | Free energy of binding | Free energy of folding | Whole dG (kcal/mol) | GC content (%) | siRNA scales | RNAxs (position) | OligoWalk (probability value) | Guide (Tm) | Passenger (Tm) | Position (siDirect) | i-Score |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
N11.2 | 11 | ATGACAAAGATCCAAATTTCAAA | UGAAAUUUGGAUCUUUGUCau | GACAAAGAUCCAAAUUUCA | 94.5 | −30 | 1.7 | −31.1 | 31.6 | 15 | 58 | 0.78544 | 0.4 | 19.2 | 38–60 | 73.6 |
N10.1 | 10 | GGCCAAACTGTCACTAAGAAATC | UUUCUUAGUGACAGUUUGGcc | CCAAACUGUCACUAAGAAA | 87.1 | −35.8 | 1.8 | −33.1 | 36.8 | 17 | 43 | 0.923617 | 11.7 | 16.7 | 23–45 | 74.5 |