Table 2 siRNAs designed in the present study for the “M” gene and various parameters
siRNA | Conserved region ID | Target sequence (21 + 2 nt) | siRNA sequence (antisense/guide) 21 nt | Sense/passenger (19 nt) | SMEpred (efficacy) | Free energy of binding | Free energy of folding | Whole dG (kcal/mol) | GC content (%) | siRNA scales | RNAxs (position) | OligoWalk (probability value) | Guide (Tm) | Passenger (Tm) | Position (siDirect) | i-Score |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
M8.5 | 8 | CACGAACGCTTTCTTATTACAAA | UGUAAUAAGAAAGCGUUCGug | CGAACGCUUUCUUAUUACA | 99.5 | −32.4 | 1.7 | −31.9 | 36.8 | 4 | 118/(98–120) | 0.808041 | 75.2 | |||
M8.3 | 8 | ATCACGAACGCTTTCTTATTACA | UAAUAAGAAAGCGUUCGUGau | CACGAACGCUUUCUUAUUA | 93.3 | −30.8 | − | − | 36.8 | 8 | 116/(96–118) | 0.880858 | 70.3 |