Table 4 Sequencing and chemistry details
S. no. | Instrument/chemical | Detail |
|---|---|---|
1 | Sequencing machine | ABI 3130 Genetic Analyzer |
2 | Chemistry cycle sequencing kit | BigDye Terminator version 3.1 |
3 | Polymer and capillary array | POP_7 pol Capillary Array |
4 | Analysis protocol | BDTv3-KB-Denovo_v 5.2 |
5 | Data analysis | SeqScape_ v 5.2 |
6 | Software reaction plate | Applied Biosystem MicroAmp Optical 96-Well Reaction Plate |