Table 1 PCR condition
Cycling condition | No. of cycles | |
|---|---|---|
Initial denaturation | 3 min at 94 °C | |
Denaturation | 1 min at 94 °C | 35 cycles |
Annealing | 1 min at 50 °C | |
Extension | 2 min at 72 °C | |
Final extension | 7 min at 72 °C | |
Cycling condition | No. of cycles | |
|---|---|---|
Initial denaturation | 3 min at 94 °C | |
Denaturation | 1 min at 94 °C | 35 cycles |
Annealing | 1 min at 50 °C | |
Extension | 2 min at 72 °C | |
Final extension | 7 min at 72 °C | |