Table 4 qRT-PCR reaction system: melting curve reaction conditions
From: The NDUFV2 gene silencing inhibits the proliferation of two drug-resistant cancer cell lines
Step | Heating rate (°C/s) | Temperature (°C) | Time (s) |
|---|---|---|---|
1 | 1.6 | 95 | 15 |
2 | 1.6 | 60 | 60 |
3 | 0.15 | 95 | 15 |