Table 3 qRT-PCR reaction system: real-time fluorescent quantitative PCR reaction conditions (standard cycle mode)
From: The NDUFV2 gene silencing inhibits the proliferation of two drug-resistant cancer cell lines
Step | Temperature (°C) | Duration (min) | Number of cycles |
|---|---|---|---|
UDG enzyme activation | 50 | 2 | - |
Pre-denaturation | 95 | 3 | - |
Denaturation | 95 | 5 | - |
Annealing | 55 | 10 | 50 |
Extension | 72 | 15 | - |