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Table 3 qRT-PCR reaction system: real-time fluorescent quantitative PCR reaction conditions (standard cycle mode)

From: The NDUFV2 gene silencing inhibits the proliferation of two drug-resistant cancer cell lines

Step Temperature (°C) Duration (min) Number of cycles
UDG enzyme activation 50 2 -
Pre-denaturation 95 3 -
Denaturation 95 5 -
Annealing 55 10 50
Extension 72 15 -