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Table 2 qRT-PCR reaction system: real-time fluorescent quantitative PCR reaction system

From: The NDUFV2 gene silencing inhibits the proliferation of two drug-resistant cancer cell lines

Reagent Volume (μL) Concentration
DEPC water 0.4 -
SYBR Green dye 10
Upstream primer (10 μM) 0.8 0.4 μM
Downstream primer (10 μM) 0.8 0.4 μM
cDNA template 2 -
Total volume 20 -