Table 2 qRT-PCR reaction system: real-time fluorescent quantitative PCR reaction system
From: The NDUFV2 gene silencing inhibits the proliferation of two drug-resistant cancer cell lines
Reagent | Volume (μL) | Concentration |
|---|---|---|
DEPC water | 0.4 | - |
SYBR Green dye | 10 | 1× |
Upstream primer (10 μM) | 0.8 | 0.4 μM |
Downstream primer (10 μM) | 0.8 | 0.4 μM |
cDNA template | 2 | - |
Total volume | 20 | - |