Structural and functional characterization of a hypothetical protein in the RD7 region in clinical isolates of Mycobacterium tuberculosis — an in silico approach to candidate vaccines

Journal of Genetic Engineering and Biotechnology

Table 1 Physical and chemical characteristics of NHP1 protein predicted using EXPASY ProtParam server

Physicochemical parameters	Values
Number of amino acids	248
Molecular weight	26331.53 Da
pH	4.90
Theoretical isoelectric point (pI)	5.09
Formula	C₁₁₆₇H₁₈₂₆N₃₂₄O₃₆₃S₄
Total number of atoms	3684
Aliphatic index	86.65
Instability index	44.06
Extinction coefficients (all pairs of Cys residues form cystines)	16055
Extinction coefficients (all Cys residues are reduced)	15930
Total number of negatively charged residues (Asp + Glu)	28
Total number of positively charged residues (Arg + Lys)	21
Grand average of hydropathicity (GRAVY)	0.035