Table 4 Kinetic parameters of SpPMO with and without inhibitors
Without inhibitor | +Niazirin (5 μM) | +Niazirin (10 μM) | +Curcumin (5 μM) | +Curcumin (10 μM) | |
|---|---|---|---|---|---|
Vmax (nmol min−1 mg−1) | 245.59 | 245.59 | 245.55 | 91.49 | 44.86 |
Km (μM) | 3.81 | 4.84 | 5.89 | 3.98 | 3.71 |
Kcat (min−1) | 14.24 | 14.24 | 14.24 | 5.306 | 2.602 |
Ki (μM) | - | 18.46 | 18.29 | 2.96 | 2.23 |