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Table 1 Cell line, bacterial strains, and plasmids used in this study

From: A small molecule, C24H17ClN4O2S, inhibits the function of the type III secretion system in Salmonella Typhimurium

Cell line, bacterial strain or plasmid

Properties

Reference or source

Cell line

  

HT29

Human colon adenocarcinoma cell line

ATCC

Salmonella Typhimurium strains

 SL1344

Wild-type

Salmonella genetic stock center

 SA

sipA::strep-tag

Current study

 SW002

invC::kanR

[20]

Escherichia coli strains

 BL21 (DE3)

F–, ompT, hsdSB (rB–, mB–), gal, dcm (DE3)

Novagen

Plasmids

 pJBT

pBAD24 derivative with C-terminal strep-tag

[21]

 pJWA

pJBT derivertive with full-length SipA

Current study

 pJWAK

pJWA derivative containing kanamycin resistance gene

Current study

 pKD13

Template plasmid for kanamycin cassette

[22]

 pKD46

Red recombinase expression plasmid

[22]

 pCP20

Flp recombinase expression plasmid

[22]

 pGEX-2T

 pGWC

Expression vector with GST fusion

pGEX-2T derivative with full-length InvC

GE Healthcare

Current study