Cell line, bacterial strain or plasmid | Properties | Reference or source |
---|---|---|
Cell line | Â | Â |
HT29 | Human colon adenocarcinoma cell line | ATCC |
Salmonella Typhimurium strains | ||
 SL1344 | Wild-type | Salmonella genetic stock center |
 SA | sipA::strep-tag | Current study |
 SW002 | invC::kanR | [20] |
Escherichia coli strains | ||
 BL21 (DE3) | F–, ompT, hsdSB (rB–, mB–), gal, dcm (DE3) | Novagen |
Plasmids | ||
 pJBT | pBAD24 derivative with C-terminal strep-tag | [21] |
 pJWA | pJBT derivertive with full-length SipA | Current study |
 pJWAK | pJWA derivative containing kanamycin resistance gene | Current study |
 pKD13 | Template plasmid for kanamycin cassette | [22] |
 pKD46 | Red recombinase expression plasmid | [22] |
 pCP20 | Flp recombinase expression plasmid | [22] |
 pGEX-2T  pGWC | Expression vector with GST fusion pGEX-2T derivative with full-length InvC | GE Healthcare Current study |