Table 2 Comparison of physicochemical parameters of nitrilase-3 protein with aliphatic and aromatic nitrilase
Sl no. | Properties | Average value for aliphatic | Average value for aromatic | Nitrilas-3 |
|---|---|---|---|---|
1 | Number of amino acids: | 330-382 | 300-330 | 266 |
2 | Molecular weight (Da) | 38,274.0 | 33,693.5 | 29,078.86 |
3 | Theoretical pI: | 5.5 | 5.5 | 4.76 |
4 | number of negatively charged residues (Asp + Glu): | 41.7 | 35.8 | 41 |
5 | number of positively charged residues (Arg + Lys): | 30.3 | 29.2 | 27 |
6 | Aliphatic index: | 89.40 | 89.90 | 89.85 |
7 | Grand average of hydropathicity (GRAVY) | 00.10 | 00.01 | -0.193 |
8 | Instability index: | 41.2 | 38.5 | 21.25 |
9 | Extinction coefficient (M−1 cm−1) at 280 nm | 50213.3 | 43975.0 | 24075 |
10 | Alanine content (%) | 13 | 9.5 | 10.20 |