Fig. 2

Expression analysis of recombinant BuLIF in Lemo-21 (DE3), A The transformed Lemo21 (DE3) competent cells containing recombinant construct were induced with 1 mM IPTG at 37 °C.SDS-PAGE analysis showed recombinant protein BuLIF/His6x was expressed at different time interval of 4 h and 12 h. An equal volume of cells were loaded onto lanes 2–5. Lane 1, protein marker; lane 2, total cell protein before IPTG induction and lanes 3, 4, and 5, total cell protein after induction at different time of interval after IPTG induction. The maximum expression of recombinant protein was observed at 12 h. B The soluble and insoluble fractions after cell lysis were observed via SDS-PAGE showed that recombinant BuLIF was expressed mainly in soluble fraction. Lane 1, insoluble fraction; lane 2, soluble fraction; lane 3, protein marker. Arrow represents the soluble fraction of recombinant BuLIF in comparison to un-induced cells after cell lysis