Fig. 1

IgG titers determined by indirect ELISA against the recombinant VgrG. The endpoint titer was defined as the highest dilution at which the optical density was 0.1 greater than that of the control wells receiving control adjuvant serum. Comparisons between the groups were performed using a Kruskal-Wallis test followed by Dunn’s multiple comparison test (**p < 01, ***p < 001)