Table 1 List of primers used
Gene | Sequence 5′ to 3′ | Accession no. | Product size (bp) | Annealing, °C |
|---|---|---|---|---|
AMH | F: CTGCTTCACACGAAAGACCC R: GCGTGAGAGTCTCCAGGAAG | XM_006047815.1 | 170 | 54 |
PLA2G3 | F: CTGTGTGGAGTGGTACTGGT R: ACTTCTGAGGATGCTGTATCA | XM_006051028.1 | 176 | 50 |
CASP3 | F: GACTGTGGTATTGAGACAGACA R: CGTACTTTTTCAGCATCTCAC | XM_006075118.1 | 175 | 50 |
PCNA | F: GTAGTAAAGATGCCTTCTGGTG R: AAGAAGTTCAGGTACCTCAGTG | XM_006047748.1 | 239 | 50 |
LHCGR | F: CATCACCTATGCTATTCAACTG R: TGGTTAAGATGTAGACCTGTGA | NM_174381.1 | 191 | 50 |
GAPDH | F: CTACATGGTCTACATGTTCCAG R: CCTTCTCCATGGTAGTGAAGA | XM_006065800.1 | 200 | 50 |
18S | F: CGCAGCTAGGAATAATGGAA R: TCTGATCGTCTTCGAACCTC | NR_036642 | 217 | 55 |
ACTB | F: GGCATTCACGAAACTACCTT R: CAATCCACACGGAGTACTTG | NM_001290932.1 | 198 | 55 |