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Table 1 Overall purification profile of cellulase from Bacillus licheniformis strain-Z9

From: Purification, biochemical characterization, and molecular cloning of cellulase from Bacillus licheniformis strain Z9 isolated from soil

Purification step Volume (ml)a Total protein (mg)b Total activity (units) Specific activity (U mg –1 Protein min−1) Purification (fold)c Yield (%)d
Crude extract 150 157.5 26700 169.5 1 100
(NH4)2SO4 Precipitate 39 40.95 13650 333.4 1.96 51.1
Dialysis 20 9.5 3300 347.3 2.04 12.35
Gel exclusion chromatography (Sephadex G-100) 15 2.3 820 356.5 2.1 3.07
  1. a,b values represent the mean of the values from three independent experiments, with a standard deviation of < ± 5%
  2. Specific activity = total activity/total protein
  3. cPurification fold = specific activity of particular purification step/specific activity of the crude enzyme
  4. dYield (%) = (total activity of the particular purification step/total activity of the crude enzyme) × 100