Purification, biochemical characterization, and molecular cloning of cellulase from Bacillus licheniformis strain Z9 isolated from soil

Journal of Genetic Engineering and Biotechnology

Table 1 Overall purification profile of cellulase from Bacillus licheniformis strain-Z9

Purification step	Volume (ml)^a	Total protein (mg)^b	Total activity (units)	Specific activity (U mg ^–1 Protein min⁻¹)	Purification (fold)^c	Yield (%)^d
Crude extract	150	157.5	26700	169.5	1	100
(NH₄)₂SO₄ Precipitate	39	40.95	13650	333.4	1.96	51.1
Dialysis	20	9.5	3300	347.3	2.04	12.35
Gel exclusion chromatography (Sephadex G-100)	15	2.3	820	356.5	2.1	3.07

^a,^b values represent the mean of the values from three independent experiments, with a standard deviation of < ± 5%
Specific activity = total activity/total protein
^cPurification fold = specific activity of particular purification step/specific activity of the crude enzyme
^dYield (%) = (total activity of the particular purification step/total activity of the crude enzyme) × 100