Table 5 Effect of incubation period on lactic acid production using immobilized cells of Enterococcus hirae-68
Incubation period (h) | Number of runs | Lactic acid (g/l) (mean ± SD) | Consumed sugar (g/l) | Yield (%) | Efficiency (%) |
|---|---|---|---|---|---|
24 | (1–18) 18 | 8 ± 0.7–10 ± 0.8 | 13–14.8 | 62–68 | 26–30 |
36 | (1) 1 | 13.5 ± 0.7 | 18.95 | 71 | 38 |
(2–3) 2 | 24.41 ± 0.41–35.5 ± 0.5 | 31.18–42.22 | 78–84 | 62–84 | |
(4–8) 5 | 31.27 ± 0.47–34.1 ± 0.4 | 36.4–40 | 86–85 | 73–80 | |
(9–10) 2 | 28.15 ± 0.15–33.03 ± 0.43 | 33.28–38.16 | 85–87 | 67–76 | |
(11–13) 3 | 35.06 ± 0.26–36.13 ± 0.93 | 42.13–40.82 | 83–89 | 84–82 | |
(14–18) 5 | 30.36 ± 0.36–34.1 ± 0.7 | 35.48–39 | 86–87 | 71–78 | |
72 | (1–3) 3 | 34.1 ± 0.9–37.11 ± 0.91 | 39.03–42.38 | 87–88 | 78–85 |
(4–6) 3 | 38.43 ± 0.43–39.5 ± 0.9 | 43.8–44.5 | 88–89 | 88–89 | |
(7–9) 3 | 36.47 ± 0.47–40.04 ± 0.54 | 41.69–45.2 | 87–89 | 83–90 | |
(10–12) 3 | 33.09 ± 0.19–35.96 ± 0.34 | 38.19–41.9 | 87–86 | 76–84 | |
(13–18) 6 | 25.3 ± 0.4–29.9 ± 0.9 | 34–37.9 | 74–79 | 68–76 | |
96 | (1–4) 4 | 37.12 ± 0.32–39 ± 0.8 | 42.29–44 | 88–89 | 85–88 |
(5–9) 5 | 40.19 ± 0.44–41 ± 0.3 | 45.09–45.8 | 89–90 | 90–92 | |
(10–13) 4 | 36.07 ± 0.77–37 ± 0.8 | 41.15–42.5 | 88–87 | 82–85 | |
(14–16) 3 | 40.24 ± 0.36–41 ± 0.4 | 45.61–46 | 88–89 | 91–92 | |
(17–18) 2 | 29.95 ± 0.95–32.27 ± 0.27 | 39.2–40.89 | 76–79 | 78–82 | |
LSD at 0.05 | 1.22 |