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Table 1 Effect of some metal ions, SDS, solvents, and inhibitors on the activity of recombinant cellSNSY enzyme activity

From: Cloning of cellulase gene using metagenomic approach of soils collected from Wadi El Natrun, an extremophilic desert valley in Egypt

Residual activity of recombinant cellSNSY activity (%)Mean ± SD

Tested

Different metal ions

Cu+2

71.32 ± 0.5

5.1.1.1. Ca+

101.42 ± 1.2

5.1.1.2. Na+

100.54 ± 1.3

5.1.1.3. Mg+2

102.1 ± 0.99

5.1.1.4. Mn+2

85.. ± 0.8

5.1.1.5. Fe+2

87.5 ± 1.34

5.1.1.6. Zn+2

99.55 ± 2.2

5.1.1.7. Control (untreated enzyme)

100 ± 0.0

 

Surfactants and solvents

5.1.1.8. SDS

87.7 ± 0.8

5.1.1.9. Methanol

94.1 ± 1.2

5.1.1.10. Ethanol

99.7 ± 0.82

5.1.1.11. Glycerol

100 ± 1.0

5.1.1.12. Isopropanol

98.2 ± 1.4

5.1.1.13. DMSO

100.6 ± 0.8

5.1.1.14. Control (untreated enzyme)

100 ± 0.0

 

Metal chelate

5.1.1.15. EDTA

95.5.5 ± 2.2

5.1.1.16. Control (untreated enzyme)

100 ± 0.0