Table 1 The primers and restriction enzymes used for PCR-RFLP genotyping
From: Edema in childhood nephrotic syndrome: possible genes–hormones interplay
SNP | Primer sequence | Size of PCR product | Enzyme | Fragments |
|---|---|---|---|---|
ANP gene (A2843G) | Forward 5′-TAGGGATGA-TCGTTGCTGACTTTG-3′ Reverse 5′-GAGTGACCTT-TTGCCTTGGATT-3′ | 125 bp | Hinf1 | 103 and 22 bp |
NPRC C (-55)A | Forward 5′-CACCGTCAATTACAAACACTTGGACAAGTCTAAC-3 Reverse 5′-CACCCTTCCTCTTTCCTCCCCACTCTTCTCTCCA-3 | 370 bp | HgaI | 177, 105, and 88 bp or 265 and 105 bp |
ANP gene (ScaI) | Forward 5′-GGT GGGAAGCAGGTGGTCAGTACTCAAGTTCAG AGGATG GGC-3′′ Reverse. 5′-CAC AAC TCC ATG GCA ACAAGA TGA CAC AAA TGC-3′. | 234 bp | ScaI | 117, 96, and 21 bp or 213 and 21 bp |