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Fig. 1 | Journal of Genetic Engineering and Biotechnology

Fig. 1

From: Edema in childhood nephrotic syndrome: possible genes–hormones interplay

Fig. 1

PCR-RFLP genotyping of ANP ScaI polymorphism. A Showing visualization of 234 bp PCR product by 2% agarose gel electrophoresis, Lane M represents DNA ladder, lanes 1-7 PCR products represented by one band at 234 bp. B Showing RFLP analysis of ANP ScaI polymorphism by 3% agarose gel electrophoresis; A1A1 genotype produces 2 fragments 213 and 21 bp, A2A2 genotype produces 3 fragments 117, 96, and 21 bp (lanes 3, 5, and 6), while A1A2 produces 4 fragments 213, 117, 96, and 21 bp (lanes 2 and 7), realize that 21 bp fragment couldn’t be visualized as it’s too light and run out the gel, lane M represents DNA ladder

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