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Table 6 Analysis of variance (ANOVA) of response surface quadratic model for the production of l-asparaginase

From: Response surface methodological optimization of l-asparaginase production from the medicinal plant endophyte Acinetobacter baumannii ZAS1

Source

Sum of squares

df

Mean square

F value

p value prob > f

 

Model

2918.75

14

208.48

165.72

< 0.0001*

Significant

A—l-asparagine

1283.65

1

1283.65

1020.3

< 0.0001*

 

B—Peptone

79.12

1

79.12

62.89

< 0.0001*

 

C—Glucose

186.15

1

186.15

147.97

< 0.0001*

 

D—Na2HPO4

290.56

1

290.56

230.96

< 0.0001*

 

AB

27.58

1

27.58

21.92

0.0003*

 

AC

1.37

1

1.37

1.09

0.3127

 

AD

1.884E−0.003

1

1.884E−0.003

1.497E−0.003

0.9696

 

BC

37.1

1

3.17

2.52

0.1335

 

BD

0.68

1

0.68

0.54

0.4735

 

CD

14.92

1

14.92

11.86

0.0036*

 

A2

343.56

1

343.56

273.09

< 0.0001*

 

B2

352.03

1

352.03

279.83

< 0.0001*

 

C2

461.83

1

461.83

367.1

< 0.0001*

 

D2

310.68

1

310.68

246.95

< 0.0001*

 

Residual

18.87

15

1.26

   

Lack of fit

10.81

10

1.08

0.67

0.7239

Not significant

Pure error

8.06

5

1.61

   

Cor total

2937.63

29

    
  1. *Statistically significant at 95% confidence level (p value < 0.05)