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Table 2 Experimental design and results of PBD

From: Response surface methodological optimization of l-asparaginase production from the medicinal plant endophyte Acinetobacter baumannii ZAS1

Run order

l-asparagine

Peptone

Glucose

Lactose

Yeast extract

NaCl

MgSO4

NA2HPO4

l-asparaginase activity U/mL )

1

− 1

1

1

− 1

1

1

− 1

− 1

6.13 ± 0.43

2

1

1

− 1

− 1

− 1

− 1

1

− 1

17.18 ± 0.21

3

− 1

− 1

− 1

− 1

− 1

− 1

− 1

− 1

1.59 ± 0.34

4

− 1

− 1

1

1

− 1

1

1

− 1

2.22 ± 0.29

5

1

− 1

− 1

1

1

− 1

1

1

16.88 ± 0.61

6

1

1

− 1

1

1

− 1

− 1

− 1

16.22 ± 0.48

7

− 1

− 1

− 1

− 1

1

− 1

1

− 1

2.34 ± 0.37

8

− 1

1

− 1

1

1

1

1

− 1

5.59 ± 0.25

9

1

1

1

− 1

− 1

1

1

− 1

16.06 ± 0.47

10

1

− 1

1

− 1

1

1

1

1

17.68 ± 0.26

11

− 1

1

1

− 1

− 1

− 1

− 1

1

7.93 ± 0.34

12

1

1

1

1

− 1

− 1

1

1

22.34 ± 0.51

13

1

− 1

− 1

− 1

− 1

1

− 1

1

16.61 ± 0.36

14

− 1

1

1

1

1

− 1

− 1

1

10.59 ± 0.42

15

1

1

− 1

− 1

1

1

− 1

1

16.84 ± 0.33

16

− 1

− 1

1

− 1

1

− 1

1

1

11.5 ± 0.28

17

1

− 1

1

1

− 1

− 1

− 1

− 1

15.61 ± 0.43

18

− 1

− 1

− 1

1

− 1

1

− 1

1

2.38 ± 0.31

19

− 1

1

− 1

1

− 1

1

1

1

9.97 ± 0.51

20

1

− 1

1

1

1

1

− 1

− 1

18.59 ± 0.60