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Journal of Genetic Engineering and Biotechnology
Fig. 1 | Journal of Genetic Engineering and Biotechnology

Fig. 1

From: Effect of Ce-doped bioactive glass/collagen/chitosan nanocomposite scaffolds on the cell morphology and proliferation of rabbit’s bone marrow mesenchymal stem cells-derived osteogenic cells

Fig. 1

Steps of BM-MSCs isolation, stage 1: Steps for dissection of Rabbit’s hind limbs (tibia and femora): (a) Animal is fixed on carton board using metal pins (arms and legs) and swabbed with 80% isopropanol then (b) the pelt was clipped and peeled back to expose the hind limbs on two sides. (c) Bones are immersed in alcohol for 15 min and then transferred to the biological safety cabinet. Stage 2: The steps of bone marrow isolation from dissected hind limbs of rabbits inside the biological safety cabinet: (a‑c) Remaining muscle tissue was removed. (d, e) The two femur and tibia heads were cut with a bone cutter. (f) A medium-filled syringe was used to flush the marrow out of the femur and tibia bones. (g) The cell suspension was transferred to15 ml centrifugation tube and centrifugation was done at 1000 rpm for 5 min. (h) Cell pellets (black arrow) were re-suspended in 1 ml complete expansion medium

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