Isolation of a bacterial strain from the gut of the fish, Systomus sarana, identification of the isolated strain, optimized production of its protease, the enzyme purification, and partial structural characterization

Dhayalan, Arul; Velramar, Balasubramanian; Govindasamy, Balasubramani; Ramalingam, Karthik Raja; Dilipkumar, Aiswarya; Pachiappan, Perumal

doi:10.1186/s43141-022-00299-3

Journal of Genetic Engineering and Biotechnology

Table 3 Composition of various experiments of the PBD for independent variables and response

From: Isolation of a bacterial strain from the gut of the fish, Systomus sarana, identification of the isolated strain, optimized production of its protease, the enzyme purification, and partial structural characterization

Run	pH	Temperature	Maltose	Beef extract	Incubation time	Protease enzyme
Run	pH	Temperature	Maltose	Beef extract	Incubation time	Experimental value	Predicted value
1	7.00	35.00	2.25	0.50	4.00	130	128.48
2	10.00	35.00	0.50	2.25	32.00	101.5	110.35
3	7.00	35.00	2.25	2.25	32.00	244.9	244.18
4	4.00	35.00	2.25	2.25	4.00	135.8	146.99
5	7.00	35.00	4.00	0.50	32.00	106	126.24
6	10.00	50.00	2.25	2.25	32.00	99	102.27
7	7.00	35.00	4.00	2.25	4.00	145	153.21
8	7.00	50.00	2.25	2.25	4.00	138	125.86
9	10.00	35.00	2.25	0.50	32.00	99	121.29
10	7.00	20.00	2.25	2.25	60.00	109.23	127.29
11	4.00	35.00	2.25	2.25	60.00	105.9	94.63
12	10.00	35.00	4.00	2.25	32.00	165.6	143.20
13	7.00	35.00	2.25	2.25	32.00	243.5	244.18
14	7.00	20.00	2.25	2.25	4.00	118	126.59
15	4.00	20.00	2.25	2.25	32.00	84	103.18
16	7.00	35.00	2.25	2.25	32.00	243.5	244.18
17	10.00	35.00	2.25	2.25	60.00	178	147.51
18	4.00	35.00	0.50	2.25	32.00	84	113.98
19	4.00	35.00	2.25	4.00	32.00	137	103.97
20	7.00	35.00	2.25	4.00	60.00	102.5	111.67
21	7.00	35.00	2.25	4.00	4.00	106	99.28
22	7.00	20.00	2.25	0.50	32.00	175	146.62
23	7.00	50.00	2.25	4.00	32.00	106	128.38
24	4.00	35.00	4.00	2.25	32.00	136	134.73
25	7.00	35.00	2.25	2.25	32.00	245	244.18
26	10.00	20.00	2.25	2.25	32.00	167	170.11
27	7.00	20.00	4.00	2.25	32.00	154	151.88
28	7.00	35.00	2.25	0.50	60.00	97.89	112.27
29	10.00	35.00	2.25	4.00	32.00	103	126.39
30	7.00	35.00	4.00	4.00	32.00	110	133.49
31	7.00	50.00	0.50	2.25	32.00	142	121.74
32	7.00	35.00	2.25	2.25	32.00	244	244.18
33	7.00	20.00	2.25	4.00	32.00	136	108.47
34	7.00	35.00	0.50	0.50	32.00	136	121.59
35	4.00	50.00	2.25	2.25	32.00	145	164.34
36	7.00	35.00	0.50	4.00	32.00	95.7	84.54
37	7.00	20.00	0.50	2.25	32.00	100	109.08
38	7.00	35.00	2.25	2.25	32.00	244.2	244.18
39	7.00	50.00	4.00	2.25	32.00	164	132.54
40	7.00	50.00	2.25	0.50	32.00	98.5	120.03
41	7.00	35.00	0.50	2.25	60.00	127	124.50
42	10.00	35.00	2.25	2.25	4.00	107	98.97
43	4.00	35.00	2.25	0.50	32.00	173	138.87
44	7.00	50.00	2.25	2.25	60.00	124	121.34
45	7.00	35.00	4.00	2.25	60.00	100	105.30
46	7.00	35.00	0.50	2.25	4.00	80	80.41

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