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Table 2 Summary of purification factors of protease by Bacillus thuringiensis (SS5)

From: Isolation of a bacterial strain from the gut of the fish, Systomus sarana, identification of the isolated strain, optimized production of its protease, the enzyme purification, and partial structural characterization

Purification steps

Protease activity(U)

Total protein (mg/ml)

Specific activity

(U/mg)

Purification fold

Yield (%)

Optimized culture crude extract

483

213

2.3

1.0

100

Ammonium sulfate precipitation

421

106

3.9

1.58

87.16

Dialysis

368

43

8.8

2.21

76.19

Sephadex G-100

245

19

12.9

2.83

50.72