Purification steps | Protease activity(U) | Total protein (mg/ml) | Specific activity (U/mg) | Purification fold | Yield (%) |
---|---|---|---|---|---|
Optimized culture crude extract | 483 | 213 | 2.3 | 1.0 | 100 |
Ammonium sulfate precipitation | 421 | 106 | 3.9 | 1.58 | 87.16 |
Dialysis | 368 | 43 | 8.8 | 2.21 | 76.19 |
Sephadex G-100 | 245 | 19 | 12.9 | 2.83 | 50.72 |