Isolation of a bacterial strain from the gut of the fish, Systomus sarana, identification of the isolated strain, optimized production of its protease, the enzyme purification, and partial structural characterization

Journal of Genetic Engineering and Biotechnology

Table 1 Analysis of variance (ANOVA) for response surface quadratic model (Box-Behnken)

Source	Sum of squares	df	Mean square	F value	p value Prob > F
Model	93,778.34	20	4688.917	8.9531	< 0.0001	Significant
A- pH	23.5225	1	23.5225	0.0449	0.8339
B- Temperature	44.65581	1	44.65581	0.0852	0.7727
C- Maltose	2872.96	1	2872.96	5.4857	0.0274
D- Beef extract	887.891	1	887.891	1.6953	0.2048
E- Incubation time	14.5924	1	14.5924	0.0278	0.8688
AB	4160.25	1	4160.25	7.9437	0.0093
AC	36.6025	1	36.6025	0.0698	0.7937
AD	400	1	400	0.7637	0.3905
AE	2545.203	1	2545.203	4.8599	0.0369
BC	256	1	256	0.4888	0.4909
BD	540.5625	1	540.5625	1.0321	0.3194
BE	6.838225	1	6.838225	0.0130	0.9099
CD	490.6225	1	490.6225	0.9368	0.3424
CE	2116	1	2116	4.0403	0.0553
DE	204.633	1	204.633	0.3907	0.5376
A²	27,991.69	1	27,991.69	53.448	< 0.0001
B²	24,876.63	1	24,876.63	47.500	< 0.0001
C²	34,419.42	1	34,419.42	65.721	< 0.0001
D²	37,710.85	1	37,710.85	72.006	< 0.0001
E²	38,409.17	1	38,409.17	73.339	< 0.0001
Residual	13092.88	25	523.715
Lack of fit	13,092.88	20	654.6438	1.8657		Not significant
Pure error	0	5	0
Cor total	106,871.2	45

SD (22.885); mean (138.948); C.V % (16.470); PRESS (52371.504); R² (0.907); adjusted R² (0.779); predicate R² (0.510); Adeq precision (10.644)