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Table 2 Optimization of fermentation parameters using response surface methodology with Box-Behnken design

From: Response surface methodology mediated optimization of Lignin peroxidase from Bacillus mycoides isolated from Simlipal Biosphere Reserve, Odisha, India

Conditions

pH

Temperature

(°C)

Incubation time (h)

H2O2 conc.

(mM)

LiP activity experimental (U/mg)

LiP activity predicted (U/mg)

1

5.5

45.0

42

0.115

28.241

27.563

2

8.0

37.5

72

0.200

19.649

19.656

3

8.0

45.0

42

0.200

37.155

36.369

4

5.5

30.0

42

0.115

7.183

6.369

5

8.0

37.5

72

0.030

16.631

15.369

6

10.5

37.5

42

0.030

27.710

26.970

7

8.0

30.0

42

0.200

9.400

9.633

8

10.5

37.5

72

0.115

27.710

25.636

9

8.0

30.0

72

0.115

13.596

13.756

10

5.5

37.5

12

0.115

21.175

22.365

11

10.5

37.5

12

0.115

34.189

40.220

12

5.5

37.5

42

0.200

33.263

33.696

13

10.5

45.0

42

0.115

30.240

30.256

14

8.0

45.0

72

0.115

20.345

21.365

15

5.5

37.5

42

0.030

39.315

39.698

16

8.0

37.5

12

0.200

45.368

45.365

17

10.5

30.0

42

0.115

55.947

54.639

18

10.5

37.5

42

0.200

37.807

36.546

19

8.0

30.0

42

0.030

33.263

33.658

20

8.0

37.5

42

0.115

27.714

26.970

21

8.0

45.0

42

0.030

13.135

13.750

22

8.0

37.5

42

0.115

25.483

26.369

23

5.5

37.5

72

0.115

25.702

25.639

24

8.0

30.0

12

0.115

31.206

30.569

25

8.0

45.0

12

0.115

16.630

16.840

26

8.0

37.5

12

0.030

37.155

36.548

27

8.0

37.5

42

0.115

24.789

25.658