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Fig. 6 | Journal of Genetic Engineering and Biotechnology

Fig. 6

From: Construction, expression, and in vitro assembly of virus-like particles of L1 protein of human papillomavirus type 52 in Escherichia coli BL21 DE3

Fig. 6

Analysis of L1-HPV52 protein production in shake flask cultivation using A SDS-PAGE of crude samples and B western blot with anti-His-AP. Both Fig. 6A and B share the same protein arrangement; lane 1: BL21 DE3 no plasmid and uninduced; lane 2: BL21 (DE3 no plasmid and induced with 0.3 mM IPTG; lane 3: BL21 DE3 harboring pETSUMO-L1HPV52 and uninduced; lane 4: BL21 DE3 harboring pETSUMO-L1HPV52 and induced with 0.3 mM IPTG; lane M molecular weight size marker (kDa). C SDS-PAGE profile of purified L1-HPV52 with a fusion tag

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