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Fig. 5 | Journal of Genetic Engineering and Biotechnology

Fig. 5

From: Construction, expression, and in vitro assembly of virus-like particles of L1 protein of human papillomavirus type 52 in Escherichia coli BL21 DE3

Fig. 5

Electrophoregram of plasmid pETSUMO-L1HPV52 extracted from E. coli BL21 DE3 expression vector before production. M: O’Gene Ruler 1kb plus DNA ladder (Thermo); code number 1–4 represent transformant’s colonies number. Only 1 colony out of 6 colonies was having pETSUMO-L1HPV52 plasmid in right direction. After colony multiplication, the 4 of those multiplied colonies were re-tested by digestion using XbaI and DNA band at 7113bp were seen on agarose gel as positive confirmation of integration into expression vector

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