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Fig. 2 | Journal of Genetic Engineering and Biotechnology

Fig. 2

From: Construction, expression, and in vitro assembly of virus-like particles of L1 protein of human papillomavirus type 52 in Escherichia coli BL21 DE3

Fig. 2

Electrophoregram of plasmid isolation and digestion as a part of transformants selection step in E. coli Mach1™ -T1R harboring pD451-MR_L1-HPV52. The DNA bands were compared to 1kb DNA ladder and code numbers 6, 8, 9, 10 represent colonies number 6, 8, 9, and 10. M: DNA Ladder 1kb (Vivantis); A: size of 1 kb DNA marker; B: uncut plasmid, which demonstrates 2 types of DNA bands, nicked and supercoiled; C: plasmid digestion using 2 digestion enzymes, EcoRI and XbaI, presentation of 2 DNA bands are well defined at 4507 and 933 bps

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