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Fig. 1 | Journal of Genetic Engineering and Biotechnology

Fig. 1

From: Sustainable and efficient protocols for in vitro germination and antioxidants production from seeds of the endangered species Araucaria araucana

Fig. 1

A Healthy piñones collected from adults trees located near Villa Pehuenia (Neuquén Province, Argentina) and entire embryos separated used for in vitro culture. B Callus formation in piñón slides (up) and isolated embryos (down); where a are callus grown in embryonic axis, b and c are slides of cotyledons and endosperm with no callus formation. Photos correspond to callus 45 days old in 60 mm Petri dishes. C Antioxidant capacity of ascorbic acid solution, embryo, and callus obtained in MS medium supplemented with 1 mg/l NAA and 1.5 mg/l BAP, in light conditions. Results obtained by DPPH assay are expressed as percentage inhibition of DPPH radical at 60 s (DP60). Different letters indicate statistically significant differences. D Effect of different concentrations of callus extract (grown in MS medium with 1.5 mg/l BAP and 1 mg/l NAA in light conditions) on VERO cells viability. Values are expressed as % with respect to control cells (100%). E Representative photos of stages of in vitro germination (left), establishment (middle) and acclimatization (right) of A. araucana seedlings

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