Fig. 1From: Sustainable and efficient protocols for in vitro germination and antioxidants production from seeds of the endangered species Araucaria araucanaA Healthy piñones collected from adults trees located near Villa Pehuenia (Neuquén Province, Argentina) and entire embryos separated used for in vitro culture. B Callus formation in piñón slides (up) and isolated embryos (down); where a are callus grown in embryonic axis, b and c are slides of cotyledons and endosperm with no callus formation. Photos correspond to callus 45 days old in 60 mm Petri dishes. C Antioxidant capacity of ascorbic acid solution, embryo, and callus obtained in MS medium supplemented with 1 mg/l NAA and 1.5 mg/l BAP, in light conditions. Results obtained by DPPH assay are expressed as percentage inhibition of DPPH radical at 60 s (DP60). Different letters indicate statistically significant differences. D Effect of different concentrations of callus extract (grown in MS medium with 1.5 mg/l BAP and 1 mg/l NAA in light conditions) on VERO cells viability. Values are expressed as % with respect to control cells (100%). E Representative photos of stages of in vitro germination (left), establishment (middle) and acclimatization (right) of A. araucana seedlingsBack to article page