Nitrogen deficiency regulates premature senescence by modulating flag leaf function, ROS homeostasis, and intercellular sugar concentration in rice during grain filling

Journal of Genetic Engineering and Biotechnology

Table 3 Difference in antioxidants enzyme activities in the flag leaves of psf mutant and its wildtype under different nitrogen supplies during grain filling

DAA	Genotype	N level	SOD activity (U mg⁻¹ FW)	POD (U mg⁻¹ FW)	CAT (μmol min⁻¹ mg⁻¹ FW)	APX (μmol min⁻¹ mg⁻¹ FW)
0	WT	HN	177.09±2.17a	23.20±1.94b	223.01±4.83a	36.95±2.30a
		MN	170.34±5.12b	31.34±5.06a	221.72±13.41b	38.01±5.00a
		LN	98.68±3.80c	33.64±1.71a	181.05±5.98c	28.35±1.07b
		Means	148.70B	29.39B	208.59A	34.44A
	psf	HN	277.83±3.72a	24.43±1.17c	251.83±3.29a	31.80±2.59a
		MN	210.08±10.22b	33.26±4.98b	244.21±10.23b	30.75±3.15a
		LN	88.96±5.24c	36.19±0.39a	103.54±2.39c	21.00±1.99b
		Means	192.29A	31.29A	199.86B	27.85B
15	WT	HN	127.63±13.18a	35.44±3.06c	274.92±12.30a	25.58±1.42a
		MN	105.74±5.87b	38.17±2.41b	181.00±6.89b	20.12±4.08b
		LN	53.43±3.11c	41.12±1.18a	138.18±14.14c	14.52±2.87c
		Means	95.60A	38.24B	198.03A	20.07A
	psf	HN	106.18±10.76a	43.92±2.97c	188.83±10.62a	21.21±0.46a
		MN	71.63±4.98b	49.00±5.01b	124.37±9.81b	18.04±2.05b
		LN	37.04±1.59c	54.45±2.22a	84.27±4.19c	11.32±1.17c
		Means	71.62B	49.12A	132.49B	16.86B
30	WT	HN	85.62±8.84a	40.99±3.12c	202.56±15.79a	13.62±1.39a
		MN	51.62±3.21b	46.21±3.14b	155.28±11.37b	10.23±1.25b
		LN	28.24±1.74c	50.10±1.74c	82.09±13.78c	9.58±1.01c
		Means	55.16A	45.77B	146.64A	11.14A
	psf	HN	53.48±4.06a	54.70±1.21c	91.13±16.00a	10.71±0.12a
		MN	30.02±6.14b	63.10±8.14b	72.51±6.77b	7.82±1.22b
		LN	21.81±2.01c	72.26±0.85a	21.72±7.22c	5.61±0.81c
		Means	35.10B	63.35A	61.79B	8.05AB
Means effect		Nitrogen	**	**	**	*
Means effect		Genotype	**	*	*	*
Interaction		N*G	*	*	*	ns

Different letters within the columns indicate a significant difference between nitrogen treatments for a genotype and mean values indicate a significant difference between the genotypes under all the treatments at p ≤ 0.05 level least significant difference (LSD)
DAA days after anthesis, HN high N, MN medium N, LN low N
** and * indicate the significance at the 0.01 and 0.05 level, respectively; NS means no significant difference based on the analysis of variance. Data are presented as means ± SD (n=3)