Nitrogen deficiency regulates premature senescence by modulating flag leaf function, ROS homeostasis, and intercellular sugar concentration in rice during grain filling

Zakari, Shamsu Ado; Zaidi, Syed Hassan Raza; Sunusi, Mustapha; Dauda, Kabiru Dawaki

doi:10.1186/s43141-021-00275-3

Journal of Genetic Engineering and Biotechnology

Table 1 Differences in the senescent-associated parameters in the flag leaves of the psf mutant and its wildtype under different N supply levels

From: Nitrogen deficiency regulates premature senescence by modulating flag leaf function, ROS homeostasis, and intercellular sugar concentration in rice during grain filling

Sampling stage	Genotype (G)	N Level (N)	Chl (a) (mg g⁻¹ FW)	Chl (b) (mg g⁻¹ FW)	SPAD	Pn (μmol CO₂ m⁻² s⁻¹)	Fv/Fm	Fm/Fo
0 DAA	Wildtype	HN	4.09±0.43a	1.89±0.19a	37.76±2.30a	17.1±1.84a	0.82±0.012a	5.54±0.20a
		MN	3.82±0.22b	1.82±0.12a	35.61±3.10a	11.1±0.08b	0.71±0.10b	5.13±0.41a
		LN	2.03±0.21c	1.26±0.18b	23.44±2.37b	10.3±0.08c	0.51±0.014c	3.51±0.24b
		Means	3.45A	1.59A	32.27A	12.85A	0.68A	4.73A
	psf	HN	4.19±0.34a	1.50±0.12a	35.70±1.26a	11.9±0.18b	0.72±0.014a	5.01±0.21a
		MN	3.61±0.18b	1.61±0.23a	30.72±1.53b	15.3±0.10a	0.57±0.03b	3.57±1.00b
		LN	1.29±0.33c	1.00±0.22b	21.01±0.98c	9.6±0.35c	0.44±0.012c	2.33±0.27c
		Means	3.03AB	1.37A	29.14B	12.31A	0.57B	3.64B
15 DAA	Wildtype	HN	3.64±0.13a	1.38±0.05a	35.41±0.35a	15.4±0.33a	0.86±0.036a	4.63±0.13a
		MN	2.05±0.25b	1.09±0.10ab	32.26±0.20a	10.0±0.38b	0.64±0.031b	3.01±0.41b
		LN	1.69±0.22c	0.92±0.05b	19.83±0.30b	5.6±0.03c	0.36±0.009c	2.36±0.09c
		Means	2.469A	1.13A	29.16A	10.39A	0.62A	3.33A
	psf	HN	3.71±0.36a	1.13±0.12b	30.87±1.17a	8.9±1.78a	0.65±0.014a	3.83±0.11a
		MN	2.16±0.31b	1.56±0.26a	21.68±2.17b	7.5±0.82b	0.42±0.03b	2.80±0.54b
		LN	0.64±0.06c	0.50±0.11c	14.51±1.15c	3.23±0.78c	0.28±0.003c	2.02±0.03c
		Means	2.17AB	1.06A	22.35B	6.57B	0.45B	2.88AB
Harvesting	Wildtype	HN	2.55±0.27a	1.29±0.12a	23.82±1.36a	10.2±1.88a	0.56±0.027a	4.02±0.17a
		MN	1.81±0.06b	1.01±0.08ab	18.11±0.20b	5.0±0.69b	0.44±0.03b	2.97±0.61b
		LN	1.18±0.11c	0.86±0.11b	10.80±1.00c	1.4±0.29c	0.36±0.010c	1.5±0.01c
		Means	1.85A	1.05A	17.57A	5.57A	0.45A	2.83A
	psf	HN	1.31±0.58a	0.81±0.19a	20.04±3.10a	4.7±0.48a	0.39±0.016a	2.22±0.01a
		MN	1.17±0.08a	0.74±0.24a	11.01±1.02b	4.0±0.81b	0.21±0.001b	1.01±0.10b
		LN	0.25±0.12b	0.24±0.04b	8.47±1.02c	1.1±0.36c	0.17±0.005	0.72±0.05c
		Means	0.91B	0.60B	13.17B	3.30B	0.25B	1.32B
Means effect		Nitrogen	*	*	*	**	**	*
Means effect		Genotype	*	ns	*	*	*	*
Interaction		N*G	ns	ns	*	*	*	ns

Different letters within the columns indicate a significant difference between nitrogen treatments for a genotype and mean values indicate a significant difference between the genotypes under all the nitrogen levels at the same sampling stage at p ≤ 0.05 using the least significant difference (LSD)
DAA days after anthesis, HN high N, MN medium N, LN low N
** and * indicate the significance at the 0.01 and 0.05 level, respectively; NS means no significant difference based on the analysis of variance. Data are presented as means ± SD (n=3)

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