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Table 2 SSCP electrophoresis conditions of the amplicons of the IGF2 gene in three lines of Japanese quails. All PCR amplicons were electrophoresed on 0.1 mm thickness of 10 Ɨ 20 (L Ɨ W) cm diameters polyacrylamide gels

From: Detection of a novel single nucleotide polymorphism in IGF2 gene with a negative impact on egg production and body weight in Japanese quail (Coturnix japonica)

Set

Amplicons

Gel concentration

Running temperature

Running time

Running voltage

Running amperage

1

IGF2,exo2

8%

16Ā°C

4.0 hr

210V

100 mA

2

IGF2,exo3

10%

18Ā°C

4.5 hr

200V

100 mA

3

IGF2,exo4

8%

20Ā°C

4.5 hr

200V

105 mA