Table 2 Embryogenic capacity, percentage of Type 3 callus, and regeneration capacity in sugarcane cultivars cultured following two callus induction protocols: MS3 (3 mg/L 2,4-D for 8 weeks) and MS3/MS1 (3 mg/L 2,4-D for 4 weeks, 1 mg/L 2,4-D for 4 weeks)
Genotype | Protocol | Embryogenic capacity (%) | Percentage of Type 3 callus* | Regeneration capacity (number of plantlets/plate) |
|---|---|---|---|---|
NA 85-1602 | MS3 | 96.00 ± 2.08 a | 3.96 ± 0.04 a | 202.50 ± 15.87 a |
FAM 81-820 | 0.00 ± 0.00 | 0.00 ± 0.00 | 00.00 ± 00.00 | |
INTA CP 98-828 | 84.50 ± 3.97 b | 4.00 ± 0.04 a | 170.86 ± 9.88 a | |
INTA NA 89-686 | 94.83 ± 2.37 a | 3.25 ± 0.04 b | 119.20 ± 16.27 b | |
INTA NA 91-209 | 79.13 ± 2.62 b | 3.16 ± 0.04 b | 135.50 ± 12.36 b | |
L 91-281 | 36.00 ± 5.29 c | 1.66 ± 0.04 e | 41.88 ± 6.72 c | |
NA 85-1602 | MS3/MS1 | 90.00 ± 2.90 a | 3.96 ± 0.04 a | 190.00 ± 12.59 a |
FAM 81-820 | 0.00 ± 0.00 | 0.00 ± 0.00 | 00.00 ± 00.00 | |
INTA CP 98-828 | 96.00 ± 2.06 a | 4.00 ± 0.04 a | 181.43 ± 12.12 a | |
INTA NA 89-686 | 89.75 ± 4.26 a | 3.26 ± 0.04 b | 130.71 ± 17.31 b | |
INTA NA 91-209 | 86.88 ± 2.62 b | 3.03 ± 0.04 c | 138.43 ± 6.99 b | |
L 91-281 | 45.25 ± 2.43 c | 1.93 ± 0.04 d | 53.00 ± 4.51 c |