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Table 2 Embryogenic capacity, percentage of Type 3 callus, and regeneration capacity in sugarcane cultivars cultured following two callus induction protocols: MS3 (3 mg/L 2,4-D for 8 weeks) and MS3/MS1 (3 mg/L 2,4-D for 4 weeks, 1 mg/L 2,4-D for 4 weeks)

From: Optimized somatic embryogenesis and plant regeneration in elite Argentinian sugarcane (Saccharum spp.) cultivars

Genotype Protocol Embryogenic capacity (%) Percentage of Type 3 callus* Regeneration capacity (number of plantlets/plate)
NA 85-1602 MS3 96.00 ± 2.08 a 3.96 ± 0.04 a 202.50 ± 15.87 a
FAM 81-820 0.00 ± 0.00 0.00 ± 0.00 00.00 ± 00.00
INTA CP 98-828 84.50 ± 3.97 b 4.00 ± 0.04 a 170.86 ± 9.88 a
INTA NA 89-686 94.83 ± 2.37 a 3.25 ± 0.04 b 119.20 ± 16.27 b
INTA NA 91-209 79.13 ± 2.62 b 3.16 ± 0.04 b 135.50 ± 12.36 b
L 91-281 36.00 ± 5.29 c 1.66 ± 0.04 e 41.88 ± 6.72 c
NA 85-1602 MS3/MS1 90.00 ± 2.90 a 3.96 ± 0.04 a 190.00 ± 12.59 a
FAM 81-820 0.00 ± 0.00 0.00 ± 0.00 00.00 ± 00.00
INTA CP 98-828 96.00 ± 2.06 a 4.00 ± 0.04 a 181.43 ± 12.12 a
INTA NA 89-686 89.75 ± 4.26 a 3.26 ± 0.04 b 130.71 ± 17.31 b
INTA NA 91-209 86.88 ± 2.62 b 3.03 ± 0.04 c 138.43 ± 6.99 b
L 91-281 45.25 ± 2.43 c 1.93 ± 0.04 d 53.00 ± 4.51 c
  1. Means followed by the same letter do not differ according to the DGC test (< 0.05)
  2. *Visual scale 1 = 0–25%; 2 = 25–50%; 3 = 50–75%; 4 = 75–100%