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Table 2 Effect of different sources and concentrations of plant growth regulators (supplemented in the MS semisolid medium) on multiple shoot initiation from organogenic calli of gerbera

From: Picloram-induced enhanced callus-mediated regeneration, acclimatization, and genetic clonality assessment of gerbera

PGRs (mg/l)

Days to shoot initiation

No. of shoots*

Shoot length (cm)*

No. of leaves*

0.5 BAP

8.7±0.3 cd

3.7±0.9 b

3.5±0.1 de

7.7±2.1 b

1 BAP

7.0±0.0 de

4.0±0.0 b

3.8±0.2 cd

8.3±0.3 b

1.5 BAP

5.7±0.3 e

4.3±0.3 ab

2.9±0.2 ef

8.7±0.7 b

0.5 kinetin

8.7±0.3 cd

3.7±0.3 b

3.3±0.2 de

8.7±0.3 b

1 kinetin

5.3±0.3 e

4.7±0.7 ab

4.5±0.1 b

9.7±1.2 b

1.5 kinetin

4.7±0.3 e

5.7±0.3 a

5.2±0.1 a

12.0±1.0 a

0.5 thidiazuron

10.3±0.7 c

3.3±0.3 b

3.1±0.1 e

7.3±0.3 b

1 thidiazuron

12.7±0.9 b

3.0±0.6 bc

4.2±0.3 bc

6.7±0.9 b

1.5 thidiazuron

13.0±1.0 b

3.3±0.7 b

3.8±0.3 cd

7.0±1.0 b

Control

25.3±1.8 a

1.7±0.3 c

2.4±0.3 f

3.3±0.7 c

  1. *Data recorded after 6 weeks of culture
  2. Data (mean±standard error) represent three replicated treatments with 20 calli (each weighing 500 mg) per replication (control = MS medium without PGR). Data for each column followed by the different letters are significantly different according to Tukey’s test at P = 0.05