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Table 2 Effect of different sources and concentrations of plant growth regulators (supplemented in the MS semisolid medium) on multiple shoot initiation from organogenic calli of gerbera

From: Picloram-induced enhanced callus-mediated regeneration, acclimatization, and genetic clonality assessment of gerbera

PGRs (mg/l) Days to shoot initiation No. of shoots* Shoot length (cm)* No. of leaves*
0.5 BAP 8.7±0.3 cd 3.7±0.9 b 3.5±0.1 de 7.7±2.1 b
1 BAP 7.0±0.0 de 4.0±0.0 b 3.8±0.2 cd 8.3±0.3 b
1.5 BAP 5.7±0.3 e 4.3±0.3 ab 2.9±0.2 ef 8.7±0.7 b
0.5 kinetin 8.7±0.3 cd 3.7±0.3 b 3.3±0.2 de 8.7±0.3 b
1 kinetin 5.3±0.3 e 4.7±0.7 ab 4.5±0.1 b 9.7±1.2 b
1.5 kinetin 4.7±0.3 e 5.7±0.3 a 5.2±0.1 a 12.0±1.0 a
0.5 thidiazuron 10.3±0.7 c 3.3±0.3 b 3.1±0.1 e 7.3±0.3 b
1 thidiazuron 12.7±0.9 b 3.0±0.6 bc 4.2±0.3 bc 6.7±0.9 b
1.5 thidiazuron 13.0±1.0 b 3.3±0.7 b 3.8±0.3 cd 7.0±1.0 b
Control 25.3±1.8 a 1.7±0.3 c 2.4±0.3 f 3.3±0.7 c
  1. *Data recorded after 6 weeks of culture
  2. Data (mean±standard error) represent three replicated treatments with 20 calli (each weighing 500 mg) per replication (control = MS medium without PGR). Data for each column followed by the different letters are significantly different according to Tukey’s test at P = 0.05