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Table 1 Effect of different sources and concentrations of plant growth regulators (supplemented in the MS semisolid medium) on induction and proliferation of callus from leaf explants of gerbera

From: Picloram-induced enhanced callus-mediated regeneration, acclimatization, and genetic clonality assessment of gerbera

PGR (mg/l)

Days to callus induction

Callus diameter (mm)*

Fresh weight (mg)*

Dry weight (mg)*

0.5 2,4-D

12.7±0.7 c

16.0±2.1 e

256.3±6.0 f

21.0±1.2 e

01 2,4-D

12.0±0.6 cd

27.7±1.5 cd

401.7±6.4 d

28.7±1.3 d

1.5 2,4-D

11.3±0.3 cd

33.0±2.6 b

471.3±7.8 c

32.3±1.2 d

0.5 NAA

22.3±1.5a

11.7±1.4 e

211.3±6.8 g

13.7±0.9 f

1 NAA

20.3±0.9 a

16.3±0.8 e

266.3±9.6 f

20.0±1.2 e

1.5 NAA

16.3±0.9 b

23.0±1.7 d

333.7±6.9 e

22.7±1.2 e

0.5 picloram

11.3±0.9 cd

28.3±1.5 bc

452.3±8.5 c

37.3±2.3 c

1 picloram

8.3±0.3 e

43.0±2.1 a

651.7±6.9 a

66.7±2.3 a

1.5 picloram

10.0±0.6 de

32.3±1.4 bc

522.3±4.6 b

45.7±2.1 b

Control

0.0±0.0 f

0.0±0.0 f

0.0±0.0 h

0.0±0.0 g

  1. *Data recorded after 6 weeks of culture
  2. Data (mean±standard error) represent three replicated treatments with 20 leaf explants per replication (control = MS medium without PGR). Data for each column followed by the different letters are significantly different according to Tukey’s test at P = 0.05