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Table 1 Effect of different sources and concentrations of plant growth regulators (supplemented in the MS semisolid medium) on induction and proliferation of callus from leaf explants of gerbera

From: Picloram-induced enhanced callus-mediated regeneration, acclimatization, and genetic clonality assessment of gerbera

PGR (mg/l) Days to callus induction Callus diameter (mm)* Fresh weight (mg)* Dry weight (mg)*
0.5 2,4-D 12.7±0.7 c 16.0±2.1 e 256.3±6.0 f 21.0±1.2 e
01 2,4-D 12.0±0.6 cd 27.7±1.5 cd 401.7±6.4 d 28.7±1.3 d
1.5 2,4-D 11.3±0.3 cd 33.0±2.6 b 471.3±7.8 c 32.3±1.2 d
0.5 NAA 22.3±1.5a 11.7±1.4 e 211.3±6.8 g 13.7±0.9 f
1 NAA 20.3±0.9 a 16.3±0.8 e 266.3±9.6 f 20.0±1.2 e
1.5 NAA 16.3±0.9 b 23.0±1.7 d 333.7±6.9 e 22.7±1.2 e
0.5 picloram 11.3±0.9 cd 28.3±1.5 bc 452.3±8.5 c 37.3±2.3 c
1 picloram 8.3±0.3 e 43.0±2.1 a 651.7±6.9 a 66.7±2.3 a
1.5 picloram 10.0±0.6 de 32.3±1.4 bc 522.3±4.6 b 45.7±2.1 b
Control 0.0±0.0 f 0.0±0.0 f 0.0±0.0 h 0.0±0.0 g
  1. *Data recorded after 6 weeks of culture
  2. Data (mean±standard error) represent three replicated treatments with 20 leaf explants per replication (control = MS medium without PGR). Data for each column followed by the different letters are significantly different according to Tukey’s test at P = 0.05