Fig. 2
Acclimatization of in vitro regenerants of gerbera. a Growth status of plantlets after 1 week of primary acclimatization in autoclaved sand (bar = 30 mm); b growth status (with the emergence of new leaves and shoots) of plantlets after 2 weeks of acclimatization in a mixture of sand, soil, cow urine, and tea leaf waste (bar = 30 mm); c growth status (with the emergence and enlargement of new leaves with elongation of shoots) of plantlets after 4 weeks of acclimatization (bar = 50 mm); d status of vegetative growth, proliferation, and flowering of acclimatized plantlet after 6 months of acclimatization in soil (bar = 100 mm); agarose gel electrophoresis of amplified ISSR fragments of regenerants (C1–C5 = regenerants from calli and the mother plant (P) exhibiting monomorphic banding pattern e 5′(AG)8T3′ and f 5′(CA)8AG3′ lane M = 100-bp ladder