Table 3 Primers codes, sequences (Y = C or T) as well as the total number of amplified alleles (total), polymorphic alleles (polym), monomorphic alleles (mono) unique alleles (unique) and % of total polymorphism induced in wild and regenerated accessions of A. fragrantissima
Primer | Sequence (5-3) | Number of amplified alleles in wild accessions | Number of amplified alleles in regenerated accessions | ||||||
|---|---|---|---|---|---|---|---|---|---|
Total | Polym | Mono | Unique | Total | Polym | Mono | Unique | ||
807 | (AG)8 T | 5 | 3 | 1 | 1 | 6 | 5 | 1 | 0 |
809 | (AG)8 G | 5 | 4 | 1 | 0 | 6 | 3 | 2 | 1 |
814 | (CT)8 TC | 5 | 3 | 1 | 1 | 7 | 6 | 1 | 0 |
825 | (AC)8 T | 4 | 2 | 1 | 1 | 5 | 4 | 1 | 0 |
834 | (AC)8 YT | 6 | 5 | 1 | 0 | 6 | 5 | 1 | 0 |
841 | (GA)8 YC | 5 | 4 | 1 | 0 | 6 | 5 | 1 | 0 |
844-B | (CT)8 GC | 6 | 4 | 1 | 1 | 6 | 3 | 2 | 1 |
17898-B | (CA)6 GT | 9 | 7 | 1 | 1 | 9 | 5 | 3 | 1 |
17899-A | (CA)6 AC | 5 | 4 | 1 | 0 | 5 | 5 | 0 | 0 |
17899-B | (CA)6 GG | 5 | 3 | 2 | 0 | 7 | 5 | 2 | 0 |
HB-08 | (GA)6 GG | 5 | 4 | 1 | 0 | 7 | 5 | 2 | 0 |
HB-09 | (GT)6 GG | 8 | 6 | 1 | 1 | 8 | 6 | 2 | 0 |
HB-10 | (GA)6 CG | 7 | 6 | 1 | 0 | 7 | 5 | 2 | 0 |
HB-11 | (GT)6 CG | 7 | 6 | 1 | 0 | 7 | 5 | 2 | 0 |
HB-12 | (CAC)3 GC | 8 | 7 | 1 | 0 | 8 | 6 | 2 | 0 |
HB-13 | (GAG)3 GC | 7 | 5 | 1 | 1 | 7 | 4 | 2 | 1 |
HB-14 | (CTC)3 GC | 6 | 5 | 1 | 0 | 6 | 4 | 2 | 0 |
HB-15 | (GTG)3 GC | 5 | 4 | 1 | 0 | 5 | 4 | 1 | 0 |
UBC-820 | (GT)8 C | 6 | 5 | 1 | 0 | Â | Â | Â | Â |
UBC-827 | (AC)8 G | 4 | 3 | 1 | 0 | Â | Â | Â | Â |
Total | 118 | 90 | 21 | 7 | 118 | 85 | 29 | 4 | |
Percentage | Â | 76.27 | 17.8 | 5.93 | Â | 72.03 | 24.58 | 3.6 | |