Fig. 5From: Expression of acid cleavable Asp-Pro linked multimeric AFP peptide in E. coliHPLC-ESI-MS of hydrolysis mixture (Intensity Solo 1.8 C18-2 2.1 × 100 mm 1.8 μm 90 Å). Gradient elution from 5 to 70% B in 25 min (A: 0.1% formic acid solution in water, B: 0.1% formic acid solution in AcN). Constant flow rate—0.25 ml/min. Complete product characteristics are given in Supplementary information (Table S1)Back to article page