Table 5 Determination of MspI cutting sites and fragment sizes for Trametes hirsuta strain 072 promoter regions
Region | No. of corresponding promoter gene of T.hirsuta | Nucleotide positions of MspI sites | Fragment size (40 and 220 bps) |
|---|---|---|---|
Promoter region | Prom-atp9 | Single cut (at383 | – |
Prom-atp6 | No cut | – | |
Prom-atp8 | Multiple cut (at 586,727,1043,1393) | 141 | |
Prom-C7V85-mgp08 | Double cut (at 1189,1548) | – | |
Prom-C7V85-mgp19 | Single cut (at 1788) | – | |
Prom-cob | Single cut (at 1118) | – | |
Prom-cox1 | Double cut(at1257,1793) | – | |
Prom-cox2 | Double cut (at 1257,1793) | – | |
Prom-cox3 | Single cut (at 534) | – | |
Prom-nad1 | No cut | – | |
Prom-nad2 | No cut | – | |
Prom-nad3 | Single cut (at 605) | – | |
Prom-nad4 | Single cut(at 290) | – | |
Prom-nad4l | Single cut(at 1962) | – | |
Prom-nad5 | Single cut (at 1695) | – | |
Prom-nad6 | Single cut (at 676) | – | |
Prom-s3 | No cut | – | |
Gene body region | Atp9 | Single cut (at 115) | – |
Atp6 | No cut | –– | |
Atp8 | No cut | – | |
Cob | No cut | – | |
Cox1 | Multiple cut (1056, 1343, 1782, 1799, 1913, 3286, 3645, 4313, 6145, 7526 | 17,114 | |
Cox2 | No cut | – | |
Cox3 | No cut | – | |
C7V85-mgp08 | Single cut (at 216) | – | |
C7V85-mgp19 | No cut | – | |
Nad1 | Single cut (948) | – | |
Nad2 | Single cut (at 112) | – | |
Nad3 | No cut | – | |
Nad4 | No cut | – | |
Nad4L | No cut | – | |
Nad5 | No cut | – | |
Nad6 | No cut | – | |
S3 | Single cut (at 127) | – |