Fig. 4

Meloidogyne chitwoodi induced proteinase inhibitor I gene expression in host tissues at different days post-infections. Housekeeping gene Actin gene was used to normalize expression of genes of interest. Relative gene expression was calculated biological replicates using the 2^△△Ct method and uninfected (control) values were subtracted from infected values. Y axis represents relative gene expressions, X axis indicates 1 dpi, 3 dpi, 7 dpi, 14 dpi, and 21 dpi following Meloidogyne chitwoodi infection. Error bars represents the standard error of the means of three replicates. Mc, Meloidogyne chitwoodi; dpi, days post infection