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Table 1 Combinations and concentrations of enzyme mixtures for protoplast isolation from Dictyopteris pacifica and Scytosiphon lomentaria

From: Protoplast isolation from Dictyopteris pacifica and Scytosiphon lomentaria, using a simple commercial enzyme preparation

Commercial enzymes Composition of enzyme mixtures
A B C D
Cellulase Onozuka RS (%) 1 1 1 1
Alginate lyase (U mL-1) 4 4 4 4
Driselase (%) 1 1
Chelation pre-treatment No No Yes Yes