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Journal of Genetic Engineering and Biotechnology

Table 1 Combinations and concentrations of enzyme mixtures for protoplast isolation from Dictyopteris pacifica and Scytosiphon lomentaria

From: Protoplast isolation from Dictyopteris pacifica and Scytosiphon lomentaria, using a simple commercial enzyme preparation

Commercial enzymes

Composition of enzyme mixtures

A

B

C

D

Cellulase Onozuka RS (%)

1

1

1

1

Alginate lyase (U mL-1)

4

4

4

4

Driselase (%)

1

–

1

–

Chelation pre-treatment

No

No

Yes

Yes

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