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Fig. 1 | Journal of Genetic Engineering and Biotechnology

Fig. 1

From: Protoplast isolation from Dictyopteris pacifica and Scytosiphon lomentaria, using a simple commercial enzyme preparation

Fig. 1

Protoplast isolation from Dictyopteris pacifica. a A germline blade producing filaments in cultures without aeration (bar = 200 μm). b Foliose thalli after 1 month in suspension culture (bar = 1 cm). c Spherical protoplasts from blade after 2 h of enzymatic digestion (bar = 100 μm). d Freshly isolated protoplasts produced from the cortex (arrowheads) and medulla (arrows) (bar = 100 μm). e True protoplasts with red chlorophyll autofluorescence under inverted fluorescence microscope showing no cell walls (bar = 10 μm). f Protoplasts with green fluorescence stained with FDA showing their viability (bar = 10 μm)

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