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Table 1 Comparison of TALEN and CRISPR/Cas9-mediated genome editing

From: TALENs—an indispensable tool in the era of CRISPR: a mini review

Recognition type DNA-Protein DNA-RNA
Target site length 30-36 bp 23 bp
Endonuclease Fok1 Cas9
Dimerization Required Not required
Off-target Low High
Design and Assembly Labour intensive Easy
Target Range Unlimited Limited by PAM
Degenerate Recognition Yes No
Specificity High, few mismatches tolerated Moderate, comparatively more mismatches tolerated
DNA methylation sensitive Yes No
Mitochondrial Genome Engineering Easy Complicated
Precision of Genome Editing High Moderate