Table 1 Comparison of TALEN and CRISPR/Cas9-mediated genome editing
From: TALENs—an indispensable tool in the era of CRISPR: a mini review
Feature | TALEN | CRISPR/Cas9 |
|---|---|---|
Recognition type | DNA-Protein | DNA-RNA |
Target site length | 30-36 bp | 23 bp |
Endonuclease | Fok1 | Cas9 |
Dimerization | Required | Not required |
Off-target | Low | High |
Design and Assembly | Labour intensive | Easy |
Target Range | Unlimited | Limited by PAM |
Degenerate Recognition | Yes | No |
Specificity | High, few mismatches tolerated | Moderate, comparatively more mismatches tolerated |
DNA methylation sensitive | Yes | No |
Mitochondrial Genome Engineering | Easy | Complicated |
Precision of Genome Editing | High | Moderate |