Fig. 1
From: Cross-reaction between mouse and rat immunoglobulin G: does it matter in sandwich ELISA?
Reaction between individual rat serum (represented as mean O.D.) and both HRP-conjugated anti-rat IgG and HRP-conjugated anti-mouse IgG: Rat sera (n = 5) were used at dilutions 1:500 and 1:1000 and both conjugated antibodies were used at dilutions 1:1000 and 1:2000. Using Student’s t-test, results at different conditions showed statistically significant differences between reactivities with the homologous compared to the heterologous sera. However, the cross-reaction between rat sera and HRP-anti mouse-IgG remained unexpectedly and unacceptably high upon increasing both sera dilution to 1:1000 and conjugate dilution to 1:2000 (levels of reaction with anti-rat IgG and anti-mouse IgG were 0.744 ±0.051 and 0.604 ±0.05, respectively, as Δ mean O.D. = 0.14; p= .004)