An immunoinformatics study: designing multivalent T-cell epitope vaccine against canine circovirus

Journal of Genetic Engineering and Biotechnology

Table 2 Epitopes selection based on Epitope prediction score and antigenicity score

Protein	DLA type	Epitope	Position of epitopes on the protein sequence	Epitope prediction score	VaxiJen antigenicity score (threshold value, 0.7)	Select/reject
Viral replicase	DLA-8803401	FMFPHMYNY	295–303	0.8249350	0.5493	Reject
		ALYRRLTLI	273–281	0.5142250	0.7123	Select
		RVFITSNRL	251–259	0.4589590	0.0434	Reject
		KVWDGGNFI	282–290	0.3829500	− 0.4171	Reject
Capsid protein	DLA-8803401	YQHLPPFRF	86–94	0.4774550	0.9208	Select
Capsid protein	DLA-8803401	YIRAKWINW	101–109	0.3914190	2.2087	Select
Viral replicase	DLA-8850101	FMFPHMYNY	295–303	0.6891070	0.5493	Reject
		RVFITSNRL	251–259	0.5503590	0.0434	Reject
		KVWDGGNFI	282–290	0.5206210	− 0.4171	Reject
Capsid protein	DLA-8850101	SVIQGANMV	212–220	0.4310240	0.5916	Reject
Viral replicase	DLA-8850801	FMFPHMYNY	295–303	0.7916770	0.5493	Reject
		AAYPETYVK	140–148	0.7143770	0.3805	Reject
		GTMNFVARR	243–251	0.6422870	0.7786	Select
		HLQGFVNLK	60–68	0.5264080	0.8062	Select
		KVWDGGNFI	282–290	0.4437560	− 0.4171	Reject
Capsid protein	DLA-8850801	FIYDPLQDR	160–168	0.6825130	− 0.0399	Reject
		HLPPFRFFK	88–96	0.6528390	− 0.2234	Reject
		TLMENVLGR	112–120	0.6319340	− 0.7453	Reject
		KLFHLRLRR	31–39	0.6040340	0.6741	Reject
		VQYDISAYI	249–257	0.5071050	0.1127	Reject