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Table. 4 Extent of polymorphism as revealed by RAPD, ISSR, DAMD and SCoT primers

From: Genetic diversity and population structure assessment using molecular markers and SPAR approach in Illicium griffithii, a medicinally important endangered species of Northeast India

Sl. No. Name of primer Primer’s sequence Total no. of band No. of poly-morphic band No. of mono-morphic band % of poly-morphic bands Resolv-ing power (Rp) PIC (Poly-morphic information content) Distance range (Jaccard's coefficient)
RAPD
 1 OPA-11 5′-CAATCGCCGT-3′ 9 8 1 88.88 4.60 0.88 0.57-1.00
 2 OPA-12 5′-TCGGCGATAG-3′ 12 12 0 100.00 5.49 0.83  
 3 OPA-13 5′-CAGCACCCAC-3′ 11 11 0 100.00 5.00 0.87  
 4 OPB-1 5′-GTTTCGCTCC-3′ 11 11 0 100.00 4.64 0.80  
 5 OPB-6 5′-TGCTCTGCCC-3′ 10 9 1 90.00 3,75 0.79  
 6 OPH-19 5′-CTGACCAGCC-3′ 13 13 0 100.00 5.32 0.76  
 7 OPK-4 5′-CCGCCCAAAC-3′ 7 7 0 100.00 2.59 0.70  
ISSR
 8 SUNSRK-5 5′-GAGAGAGAGAGAGAGATT-3′ 9 9 0 100.00 3.93 0.86  
 9 SUNSRK-6 5′-CTCTCTCTCTCTCTCTTA-3′ 12 12 0 100.00 6.03 0.88  
 10 SUNSRK-11 5′-GGCGGCGGCGGCGGCGGC-3′ 8 8 0 100.00 4.45 0.89  
 11 SUNSRK-14 5′-CACACACACACAAC-3′ 10 10 0 100.00 6.34 0.88  
 12 SUNSRK-14 5′-CACACACACACAGG-3′ 7 7 0 100.00 5.77 0.83  
 13 SUNSRK-21 5′-ACACACACACACACACAG-3′ 8 8 0 100.00 4.95 0.74  
 14 SUNSRK-22 5′-ACACACACACACACACT-3′ 7 6 1 85.71 2.66 0.70  
DAMD
 15 URP 13R 5′-TACACGTCTCGATCTACA-3′ 5 5 0 100.00 2.45 0.87  
 16 Oligo 5 5′-GACNGGNACNGG-3′ 10 10 0 100.00 2.97 0.95  
 17 URP 38F 5′-AAGAGGCATTCTACCACC-3′ 6 6 0 100.00 3.40 0.88  
 18 URP 9R 5′-ATGTGTGCGATCAGTTGC-3′ 7 7 0 100.00 4.57 0.79  
 19 HBV 3 5′-GGTGAAGCSCAGGTG-3′ 9 9 0 100.00 3.94 0.73  
 20 URP 25F 5′-GATGTGTTCTTGGAGCCT-3′ 8 8 0 100.00 4.53 0.79  
 21 Oligo 2 5′-CTCTGGGTGTCGTGC-3′ 9 9 0 100.00 5.60 0.69  
 22 HVY 5′-GCCTTTCCCGAG-3′ 8 8 0 100.00 4.33 0.69  
SCoT
 23 SCOT-1 5′-CAACAATGGCTACCACCA-3′ 8 8 0 100.00 3.56 0.84  
 24 SCOT-2 5′-CAACAATGGCTACCACCC-3′ 8 8 0 100.00 3.30 0.90  
 25 SCOT-13 5′-ACGACATGGCGACCATCG-3′ 8 8 0 100.00 4.57 0.86  
 26 SCOT-14 5′-ACGACATGGCGACCACGC-3′ 10 10 0 100.00 4.41 0.84  
 27 SCOT-22 5′-AACCATGGCTACCACCAC-3′ 10 10 0 100.00 4.57 0.80  
 28 SCOT-23 5′-CACCATGGCTACCACCAG-3′ 10 9 1 90.00 3.40 0.76  
   Total 250 246 4 98.40    
   Average 08.92 08.78 0.14