Programme | Initial denaturation | PCR cycle | Final extension | No. of cycles | ||
---|---|---|---|---|---|---|
RAPD | 94 °C (3 min) | 94 °C (45 s) | 36 °C (1 min) | 72 °C (2 min) | 72 °C (7 min) | 45 |
ISSR | 94 °C (3 min) | 92 °C (2 min) | 38–54 °C (1 min) | 72 °C (2 min) | 72 °C (7 min) | 40 |
DAMD | 94 °C (4 min) | 94 °C (1 min) | 50 °C (2 min) | 72 °C (2 min) | 72 °C (7 min) | 40 |
SCoT | 95 °C (5 min) | 94 °C (45 s) | 55 °C (1 min) | 72 °C (2 min) | 72 °C (7 min) | 40 |