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Table. 2 Reaction settings for the PCR cycles

From: Genetic diversity and population structure assessment using molecular markers and SPAR approach in Illicium griffithii, a medicinally important endangered species of Northeast India

Programme Initial denaturation PCR cycle Final extension No. of cycles
RAPD 94 °C (3 min) 94 °C (45 s) 36 °C (1 min) 72 °C (2 min) 72 °C (7 min) 45
ISSR 94 °C (3 min) 92 °C (2 min) 38–54 °C (1 min) 72 °C (2 min) 72 °C (7 min) 40
DAMD 94 °C (4 min) 94 °C (1 min) 50 °C (2 min) 72 °C (2 min) 72 °C (7 min) 40
SCoT 95 °C (5 min) 94 °C (45 s) 55 °C (1 min) 72 °C (2 min) 72 °C (7 min) 40