Skip to main content

Table. 2 Reaction settings for the PCR cycles

From: Genetic diversity and population structure assessment using molecular markers and SPAR approach in Illicium griffithii, a medicinally important endangered species of Northeast India

Programme

Initial denaturation

PCR cycle

Final extension

No. of cycles

RAPD

94 °C (3 min)

94 °C (45 s)

36 °C (1 min)

72 °C (2 min)

72 °C (7 min)

45

ISSR

94 °C (3 min)

92 °C (2 min)

38–54 °C (1 min)

72 °C (2 min)

72 °C (7 min)

40

DAMD

94 °C (4 min)

94 °C (1 min)

50 °C (2 min)

72 °C (2 min)

72 °C (7 min)

40

SCoT

95 °C (5 min)

94 °C (45 s)

55 °C (1 min)

72 °C (2 min)

72 °C (7 min)

40