Table 3 List of RAPD and ISSR primers’ names, sequences, annealing temperatures, bands sizes range, and number of bands amplified including monomorphic (MB) and polymorphic bands (PB)
Molecular marker | Primer name | Primer sequence 5′ to 3′ | Temp. (°C) | Size range (bp) | Total amplified bands | MB | PB |
|---|---|---|---|---|---|---|---|
RAPD | OPA-03 | AGTCAGCCAC | 39.5 | 1787–921 | 4 | 4 | 0 |
OPA-04 | AATCGGGCTG | 39.5 | 2667–408 | 13 | 12 | 1 | |
OPH-07 | CTGCATCGTG | 39.5 | 2200–613 | 6 | 6 | 0 | |
OPI-07 | CAGCGACAAG | 39.5 | 1926–617 | 4 | 3 | 1 | |
OPK-07 | AGCGAGCAAG | 39.5 | 1788–690 | 11 | 11 | 0 | |
OPM-07 | CCGTGACTCA | 39.5 | 3860–840 | 7 | 7 | 0 | |
OPO-07 | CAGCACTGAC | 39.5 | 1642–538 | 4 | 4 | 0 | |
OPS-07 | TCCGATGCTG | 39.5 | 1813–516 | 4 | 4 | 0 | |
OPW-07 | CTGGACGTCA | 39.5 | 2114–625 | 6 | 6 | 0 | |
OPY-07 | AGAGCCGTCA | 39.5 | 1919–621 | 4 | 4 | 0 | |
Total | Â | 63 | 61 | 2 | |||
Range |  | 3860–408 | 13–4 | 12–4 | 2–0 | ||
Average | Â | Â | 6.3 | 6.1 | 0.2 | ||
ISSR | UBC-842C | GAGAGAGAGAGAGAGACG | 59.9 | 2013–510 | 7 | 7 | 0 |
UBC-842T | GAGAGAGAGAGAGAGATG | 57.6 | 1617–567 | 9 | 7 | 2 | |
UBC-846A | CACACACACACACACACAAT | 55.3 | 1717–520 | 4 | 3 | 1 | |
UBC-849C | GTGTGTGTGTGTGTGTCA | 57.6 | 2682–678 | 13 | 12 | 1 | |
R(CA)7 | GCACACACACACACA | 53.4 | 1410–580 | 8 | 8 | 0 | |
UBC-834C | AGAGAGAGAGAGAGAGCT | 57.6 | 4220–734 | 5 | 4 | 1 | |
UBC-834T | AGAGAGAGAGAGAGAGTT | 55.3 | 1111–464 | 3 | 3 | 0 | |
UBC-848A | CACACACACACACACACAAG | 57.6 | 1450–687 | 3 | 3 | 0 | |
UBC-860A | TGTGTGTGTGTGTGTGAA | 55.3 | 2170–730 | 5 | 4 | 1 | |
(AC)8TG | ACACACACACACACACTC | 57.6 | 2738–1540 | 9 | 8 | 1 | |
Total | Â | 66 | 59 | 7 | |||
Range |  | 4220–464 | 13–3 | 12–3 | 1–0 | ||
Average | Â | Â | 6.6 | 5.9 | 0.7 | ||