Skip to main content

Table 2 Effect of different cytokinin (BAP and Kn) concentrations in combination with 0.05 mg/L NAA on shoot multiplication from different explant sources of A. pubescens

From: Metabolic profiling, in vitro propagation, and genetic assessment of the endangered rare plant Anarrhinum pubescens

Growth regulators (mg /L)

Explant source

BAP

Kn

NAA

Nodal cuttings

Leaf segments

Shoot response* (%)

No. of shoots*

Shoot response* (%)

No. of shoots*

0.5

–

0.05

100a

13.85 ± 0.7c

81.5ab

9.3 ± 0.6a

1

–

0.05

100 a

27.67 ± 1.4a

88.8ab

7.1 ± 0.7b

2

–

0.05

100 a

23.19 ± 0.5b

77.8b

7.4 ± 0.2b

3

–

0.05

100 a

13.33 ± 1.6c

48.2c

3.7 ± 0.6c

4

–

0.05

100 a

12.67 ± 1.7c

85.2ab

8.2 ± 0.2ab

–

0.5

0.05

96.3 a

3.6 ± 0.2e

74c

1.2 ± 0.6d

–

1

0.05

100 a

5.8 ± 0.6de

7.4d

0.4 ± 0.3d

–

2

0.05

100 a

8.3 ± 1.7d

66.7c

2.3 ± 0.2cd

–

3

0.05

74.1b

5.8 ± 2.4de

70.4c

1.2 ± 0.4d

–

4

0.05

92.6a

5.1 ± 0.8de

90.3a

6.5 ± 1.4b

  1. *Values represent mean ± SEM; numbers in columns followed by same letters are not significantly different according to Fisher pairwise comparison (p ≤ 0.05)